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American Society of Microbiology ABMM Sample Questions:

1. A patient develops a febrile illness with a diffuse petechial rash after a camping trip. Serological testing reveals a four-fold rise in antibody titer to Rickettsia rickettsii. The vector responsible for transmitting this organism is a:

A) Flea
B) Louse
C) Tick
D) Mosquito

2. During the processing of stool specimens for ova and parasite examination, the formalin-ethyl acetate concentration procedure primarily serves to:

A) Preserve parasite morphology and kill bacteria
B) Separate parasites from fecal debris and concentrate them
C) Selectively culture protozoan trophozoites
D) Stain parasite nuclei for easier identification

3. A patient with a history of recurrent sinusitis and bronchitis is diagnosed with cystic fibrosis. Sputum cultures repeatedly grow a Gram-negative rod that is oxidase-positive and produces a blue-green pigment. This organism's chronic colonization of the respiratory tract in cystic fibrosis patients is primarily due to its ability to:

A) Evade phagocytosis by alveolar macrophages through capsule production.
B) Produce potent exotoxins that damage the ciliated epithelium.
C) Exhibit rapid intracellular replication within respiratory epithelial cells.
D) Form a biofilm that protects it from host defenses and antibiotics.

4. A clinical microbiology laboratory is using CRISPR-based technology for rapid and sensitive detection of specific bacterial DNA sequences directly from patient samples. This method relies on the ability of the Cas enzyme to:

A) Reverse transcribe RNA into complementary DNA.
B) Specifically bind to and cleave target DNA sequences guided by a short RNA molecule.
C) Detect the presence of specific antibodies produced in response to bacterial infection.
D) Amplify target DNA sequences using specific primers and thermal cycling.

5. A patient undergoing chemotherapy for leukemia develops a severe pneumonia. Bronchoalveolar lavage fluid is examined, revealing numerous cysts containing crescent-shaped structures upon silver staining. The patient's CD4+ T-cell count is significantly low. The MOST likely diagnosis is infection with:

A) Blastomyces dermatitidis
B) Histoplasma capsulatum
C) Coccidioides immitis
D) Pneumocystis jirovecii

Solutions: